Investigation of Soil Microbial Communities Involved in N Cycling as Affected by the Long-Term Use of the N Stabilizers DMPP and NBPT

Both, 3,4-dimethylpyrazole phosphate (DMPP) and N-(n-butyl) thiophosphoric triamide (NBPT) are commonly used as nitrogen (N) stabilizers, often in agriculture to reduce loss from soils by inhibiting soil nitrification slowing down urea hydrolysis, respectively. The current knowledge gap concerns how microbial communities involved N cycling affected the long-term use of DMPP NBPT. present field study explored inter-annual variation nitrous oxide (N2O) emissions responses ammonia oxidizers (AOA, AOB encoded amoA gene), nitrite-oxidizing bacteria (NOB, nxrA nxrB genes), denitrifier (encoded narG nosZ genes) populations following a (8 years) addition results showed that reduction N2O NBPT increased year on year. population diversity significantly (p < 0.05) after application but decreased addition. potential rate (PNR) enrichment genera with high oxidation capacity population. In contrast, weakened this effect formed low capacity. Variations NOB were mainly associated fertilizer-induced changes substrate NO2−, whereas had minor impacts Additionally, change denitrification was indirectly ammonium (NH4+) content stabilizer application. These findings provide new interpretation related response mechanisms nitrifier for stabilizers soils.